The bicipital groove along the humerus is best examined from behind the patient. On one side, locate the greater tuberosity by palpating inferiorly from the acromion. Keep the fingers of one hand at this level and use the other to externally rotate the arm at the shoulder. As the arm rotates, sequentially the greater tuberosity, bicipital groove and lesser tuberosity are felt.
The groove is palpated lightly as excessive pressure will cause pain. Even light palpation may cause pain if there is bicipital tendonitis affecting the long head of biceps.
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